srt2104 srt Search Results


srt2104  (MedChemExpress)
94
MedChemExpress srt2104
Treatment with antioxidants or SIRT1 activator alleviates senescence phenotypes in hPSC-derived astrocytes . ( A ) Treatment of hPSC-derived astrocytes with antioxidant cocktail (MitoTEMPOL, MT and N-acetyl cysteine, NAC) for a week or SIRT1 activator <t>(SRT2104)</t> for 72 h significantly increased mitochondrial membrane potential. ( B ) Total ROS level was not significantly changed by the treatments, although there was a decrement tendency in the antioxidant cocktail-treated group. ( C ) Mitochondrial ROS level was not significantly affected when comparing the control group with the treatment groups. Each dot in (A)-(C) represents the value measured in each batch of differentiation. MFI: mean fluorescence intensity. The values are expressed relative to the MFI of untreated 6-week-old astrocytes (6W Ctrl). ( D ) Representative fluorescent images of astrocytes stained with MitoTracker™ Red. Scale bar, 10 µm. (E-I) Quantification and comparison of various morphological parameters of mitochondria between 6-week-old astrocytes (control group) and those that received treatments: mean area (E) , mean perimeter (F) , mean aspect ratio (G) , mean form factor (H) , mean branch length (I) . The treatment with SRT2104 significantly impacted all the parameters measured while that of an antioxidant cocktail showed no significant effect. Quantification in (E)-(I) was conducted using thirty immunofluorescent images obtained from three independent differentiations. Each dot represents the value of an individual cell, and the number of cells used in the assessment is indicated in each graph. ( J ) Treatment with either an antioxidant cocktail or SRT2104 significantly reduced the number of SA-β-Gal-positive cells compared with that of the non-treated control group. Quantification was based on twelve immunofluorescent images acquired from three batches of differentiation. Each dot represents the mean percentage of positive cells within an image. ( K ) Astrocytes treated with the antioxidant cocktail for a week or with the SIRT1 activator for 72 hours exhibited a significant increase in their ability to uptake Aβ aggregates compared to untreated 6-week-old astrocytes. Data were obtained from seven batches of differentiation. Each dot represents the value measured in an image, and the number of images used in the measurement is indicated in each graph. ns: not significant, *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.
Srt2104, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srt2104+srt/SRT+2104/pmc12096905-56-88-89
Average 94 stars, based on 1 article reviews
srt2104 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
srt2104  (TargetMol)
92
TargetMol srt2104
Figure 2. The effects of <t>mEV-SRT2104</t> on osteoblasts and osteoclasts in vitro. (A) Schematic
Srt2104, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/srt2104+srt/SRT+2104/pm39323741-46-10-16
Average 92 stars, based on 1 article reviews
srt2104 - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
srt 2104  (Cayman Chemical)
N/A
SRT 2104
   Buy from Supplier

Image Search Results


Treatment with antioxidants or SIRT1 activator alleviates senescence phenotypes in hPSC-derived astrocytes . ( A ) Treatment of hPSC-derived astrocytes with antioxidant cocktail (MitoTEMPOL, MT and N-acetyl cysteine, NAC) for a week or SIRT1 activator (SRT2104) for 72 h significantly increased mitochondrial membrane potential. ( B ) Total ROS level was not significantly changed by the treatments, although there was a decrement tendency in the antioxidant cocktail-treated group. ( C ) Mitochondrial ROS level was not significantly affected when comparing the control group with the treatment groups. Each dot in (A)-(C) represents the value measured in each batch of differentiation. MFI: mean fluorescence intensity. The values are expressed relative to the MFI of untreated 6-week-old astrocytes (6W Ctrl). ( D ) Representative fluorescent images of astrocytes stained with MitoTracker™ Red. Scale bar, 10 µm. (E-I) Quantification and comparison of various morphological parameters of mitochondria between 6-week-old astrocytes (control group) and those that received treatments: mean area (E) , mean perimeter (F) , mean aspect ratio (G) , mean form factor (H) , mean branch length (I) . The treatment with SRT2104 significantly impacted all the parameters measured while that of an antioxidant cocktail showed no significant effect. Quantification in (E)-(I) was conducted using thirty immunofluorescent images obtained from three independent differentiations. Each dot represents the value of an individual cell, and the number of cells used in the assessment is indicated in each graph. ( J ) Treatment with either an antioxidant cocktail or SRT2104 significantly reduced the number of SA-β-Gal-positive cells compared with that of the non-treated control group. Quantification was based on twelve immunofluorescent images acquired from three batches of differentiation. Each dot represents the mean percentage of positive cells within an image. ( K ) Astrocytes treated with the antioxidant cocktail for a week or with the SIRT1 activator for 72 hours exhibited a significant increase in their ability to uptake Aβ aggregates compared to untreated 6-week-old astrocytes. Data were obtained from seven batches of differentiation. Each dot represents the value measured in an image, and the number of images used in the measurement is indicated in each graph. ns: not significant, *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.

Journal: Aging and Disease

Article Title: Senescent Astrocytes Derived from Human Pluripotent Stem Cells Reveal Age-Related Changes and Implications for Neurodegeneration

doi: 10.14336/AD.2024.0089

Figure Lengend Snippet: Treatment with antioxidants or SIRT1 activator alleviates senescence phenotypes in hPSC-derived astrocytes . ( A ) Treatment of hPSC-derived astrocytes with antioxidant cocktail (MitoTEMPOL, MT and N-acetyl cysteine, NAC) for a week or SIRT1 activator (SRT2104) for 72 h significantly increased mitochondrial membrane potential. ( B ) Total ROS level was not significantly changed by the treatments, although there was a decrement tendency in the antioxidant cocktail-treated group. ( C ) Mitochondrial ROS level was not significantly affected when comparing the control group with the treatment groups. Each dot in (A)-(C) represents the value measured in each batch of differentiation. MFI: mean fluorescence intensity. The values are expressed relative to the MFI of untreated 6-week-old astrocytes (6W Ctrl). ( D ) Representative fluorescent images of astrocytes stained with MitoTracker™ Red. Scale bar, 10 µm. (E-I) Quantification and comparison of various morphological parameters of mitochondria between 6-week-old astrocytes (control group) and those that received treatments: mean area (E) , mean perimeter (F) , mean aspect ratio (G) , mean form factor (H) , mean branch length (I) . The treatment with SRT2104 significantly impacted all the parameters measured while that of an antioxidant cocktail showed no significant effect. Quantification in (E)-(I) was conducted using thirty immunofluorescent images obtained from three independent differentiations. Each dot represents the value of an individual cell, and the number of cells used in the assessment is indicated in each graph. ( J ) Treatment with either an antioxidant cocktail or SRT2104 significantly reduced the number of SA-β-Gal-positive cells compared with that of the non-treated control group. Quantification was based on twelve immunofluorescent images acquired from three batches of differentiation. Each dot represents the mean percentage of positive cells within an image. ( K ) Astrocytes treated with the antioxidant cocktail for a week or with the SIRT1 activator for 72 hours exhibited a significant increase in their ability to uptake Aβ aggregates compared to untreated 6-week-old astrocytes. Data were obtained from seven batches of differentiation. Each dot represents the value measured in an image, and the number of images used in the measurement is indicated in each graph. ns: not significant, *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001.

Article Snippet: To assess their response to a mixture of inflammatory cytokines, astrocytes at the third and sixth weeks were exposed to 30 ng/mL tumor necrosis factor α (TNFα) (Cell Signaling Technology, MA, USA), 3 ng/mL interleukin-1α (IL-1α) (PeproTech), and 400 ng/mL Complement component 1q (C1q) (Millipore Sigma) for 72 h. In certain experiments, astrocytes between the fifth and sixth weeks were treated with an antioxidant cocktail (10 nM MitoTEMPOL [MT; Cayman Chemical, MI, USA] and 30 uM N-acetyl cysteine [NAC; Millipore Sigma]) for 1 week or with 10 uM SRT2104 (MedChemExpress, NJ, USA) for 3 days.

Techniques: Derivative Assay, Membrane, Control, Fluorescence, Staining, Comparison

Figure 2. The effects of mEV-SRT2104 on osteoblasts and osteoclasts in vitro. (A) Schematic

Journal: Regenerative biomaterials

Article Title: Bone-targeting engineered milk-derived extracellular vesicles for MRI-assisted therapy of osteoporosis.

doi: 10.1093/rb/rbae112

Figure Lengend Snippet: Figure 2. The effects of mEV-SRT2104 on osteoblasts and osteoclasts in vitro. (A) Schematic

Article Snippet: The sequence is FITC-Ahx-{Lys(N3)}DSSDSSDSSDSSDSSDSS.) was purchased from IGE Biotechnology Ltd. SRT2104 (T6679, China) was purchased from TargetMol Chemicals Inc. Antibodies used in this study were rabbit polyclonal antibody against Alix (92800, Cell Signaling Technology), CD81 (27855, Proteintech), CD63 (25682, Proteintech), GAPDH (2118, Cell Signaling Technology), RUNX2 (20700, Proteintech), Calnexin (ab75801, Abcam), CTSK (ab187647, Abcam) and OPN (ab283656, Abcam).

Techniques: In Vitro